Rotational multiphoton endoscopy with a 1 m fiber laser system

نویسندگان

  • Gangjun Liu
  • Tuqiang Xie
  • Ivan V. Tomov
  • Jianping Su
  • Lingfeng Yu
  • Jun Zhang
  • Bruce J. Tromberg
  • Zhongping Chen
چکیده

We present multiphoton microendoscopy with a rotational probe and a 1 m fiber-based femtosecond laser. The rotational probe is based on a double-clad photonic crystal fiber, a gradient index lens, a microprism, and a rotational microelectronicmechanical system (MEMS) motor. The MEMS motor has a diameter of 2.2 mm and can provide 360° full-view rotation. The fiber laser provides ultrashort pulses with a central wavelength at 1.034 m and a repetition rate of 50 MHz. Second-harmonic-generation images of rat-tail tendon and fish scale are demonstrated with the rotational probe-based multiphoton system. © 2009 Optical Society of America OCIS codes: 190.4180, 170.2150, 180.5810.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Beam-scanning Multiphoton Multimodality Endoscopy

Two-photon-exciter fluorescence (2PEF) imaging and second-harmonic generation (SHG) microscopy has become well-established techniques for biomedical imaging [1]. Integration of fibers into the multiphoton microscopy system further enables in vivo and noninvasive investigation of biological samples. Nonetheless the broadening of ultrashort pulses in fibers has greatly hampered the development of...

متن کامل

Multiphoton endoscopy.

Despite widespread use of multiphoton fluorescence microscopy, development of endoscopes for nonlinear optical imaging has been stymied by the degradation of ultrashort excitation pulses that occurs within optical fiber as a result of the combined effects of group-velocity dispersion and self-phase modulation. We introduce microendoscopes (350-1000 microm in diameter) based on gradient-index mi...

متن کامل

Reduction of self-phase modulation in double-clad photonic crystal fiber for nonlinear optical endoscopy.

Double-clad photonic crystal fiber and double-clad fiber have been widely used in multiphoton-excited fluorescence or second-harmonic generation (SHG) endoscopy. We provide a useful comparison of two fibers used in nonlinear optical microendoscopy. While a double-clad fiber is found to have a higher percentage of the output power from its core, which results in the efficient utilization of the ...

متن کامل

Design and implementation of fiber-based multiphoton endoscopy with microelectromechanical systems scanning.

A multiphoton endoscopy system has been developed using a two-axis microelectromechanical systems (MEMS) mirror and double-cladding photonic crystal fiber (DCPCF). The MEMS mirror has a 2-mm-diam, 20-deg optical scanning angle, and 1.26-kHz and 780-Hz resonance frequencies on the x and y axes. The maximum number of resolvable focal spots of the MEMS scanner is 720 x 720 on the x and y axes, whi...

متن کامل

Multiphoton gradient index endoscopy for evaluation of diseased human prostatic tissue ex vivo.

Multiphoton microscopy can instantly visualize cellular details in unstained tissues. Multiphoton probes with clinical potential have been developed. This study evaluates the suitability of multiphoton gradient index (GRIN) endoscopy as a diagnostic tool for prostatic tissue. A portable and compact multiphoton endoscope based on a 1-mm diameter, 8-cm length GRIN lens system probe was used. Fres...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2009